Certificate Of Analysis
Product Name Anti-Histone Deacetylase 4 (HDAC4) (DM-15) antibody produced in rabbit,
affinity isolated antibody, buffered aqueous solution
Product Number H9536
Product Brand SIGMA
 
TEST SPECIFICATION LOT 082K4810 RESULTS
APPEARANCE CLEAR COLORLESS SOLUTION IN 0.01 M PHOSPHATE BUFFERED SALINE, PH 7.4, CONTAINING 15 MM SODIUM AZIDE AND 1% BSA
PROTEIN BY UV ABSORBANCE 1.0 MG/ML
WORKING DILUTION BY IN -DIRECT IMMUNOBLOTTING 1:2,000 USING WHOLE EXTRACTS OF MOUSE NIH-3T3 FIBROBLASTS AND CHEMILUMINESCENT DETECTION
WORKING DILUTION BY IN -DIRECT IMMUNOBLOTTING 1:1,000 USING WHOLE EXTRACTS OF RAT BRAIN AND CHEMILUMINESCENT DETECTION
IMMUNOPRECIPITATION 20 UG IMMUNOPRECIPITATES HDAC4 FROM A RIPA EXTRACT OF HELA NUCLEI FROM 1 X 10(7) CELLS
WORKING DILUTION- DIRE CT IMMUNOFLUORESCENCE 1:500 USING HEK 293T CELLS EXPRESSING RECOMBINANT MOUSE HDAC4.
QC RELEASE DATE SEPTEMBER 2002