Certificate Of Analysis
Product Name Anti-Insulin-Like Growth Factor-II antibody produced in goat,
affinity isolated antibody, lyophilized powder
Product Number I2282
Product Brand SIGMA
 
TEST SPECIFICATION LOT 047K0725 RESULTS
APPEARANCE LYOPHILIZED POWDER
BIOACTIVITY (NEUTRALIZATION ASSAY MCF-7 CELLS) ND50: 1.5-4.5 UG/ML IN PRESENCE OF 30 NG/ML RECOMBINANT MOUSE IGF-II
WORKING DILUTION BY IN -DIRECT IMMUNOBLOTTING 0.1-0.2 UG DETECTS RECOMBINANT MOUSE IGF-II AT APPROX. 25 NG/LANE UNDER NON-REDUCING AND 5 NG/LANE UNDER REDUCING CONDITIONS
WORKING DILUTION BY ELISA 0.5-1.0 UG/ML DETECTS RECOMBINANT HUMAN IGF-II AT APPROX 0.5 NG/WELL
ENDOTOXIN ASSAY < 0.1 EU/UG ANTIBODY
NOTE LYOPHILIZED FROM 0.2 UM-FILTERED SOLUTION OF PHOSPHATE BUFFERED SALINE WITH 5% TREHALOSE
QC RELEASE DATE APRIL 2007