Certificate Of Analysis
Product Name Anti-phospho-PKB (pSer473) antibody produced in rabbit,
IgG fraction of antiserum, buffered aqueous solution
Product Number P4112
Product Brand SIGMA
 
TEST SPECIFICATION LOT 127K4757 RESULTS
APPEARANCE CLEAR COLORLESS SOLUTION IM 0.01 M PHOSPHATE BUFFERED SALINE, PH 7.4, CONTAINING 15 MM SODIUM AZIDE
PROTEIN BY UV ABSORBANCE 4.6 MG/ML
IDENTITY BY IMMUNO- ELECTROPHORESIS SINGLE ARC OF PRECIPITATION VERSUS NORMAL RABBIT SERUM AND ANTI-RABBIT IGG
WORKING DILUTION BY DIRECT IMMUNOBLOTTING 1:2000 USING A WHOLE CELL EXTRACT OF PDGF-TREATED MOUSE FIBROBLASTS NIH3T3 CELL LINE
WORKING DILUTION BY DIRECT IMMUNOBLOTTING 1:1000 USING A WHOLE CELL EXTRACT OF H2O2-TREATED RAT FIBROBLASTS RAT-1 CELL LINE
QC RELEASE DATE DECEMBER 2007