Certificate Of Analysis
Product Name Monoclonal Anti-AKT3 antibody produced in mouse,
clone 6E11, purified immunoglobulin, buffered aqueous solution
Product Number SAB1404769
Product Brand SIGMA
 
TEST SPECIFICATION LOT 09141-6E11 RESULTS
Concentration 0.43 mg/mL
Isotype IgG2a Kappa
Western Blotting (Cell lysate) Antibody concentration: 1 ~ 5 ug/ml. Specific band of ~55.8 kDa using HeLa lysate. pass
Sandwich ELISA Detection limit for recombinant GST tagged AKT3 is approximately 0.03ng/ml. pass
ELISA (Recombinant protein) Antibody concentration: 1 ug/ml using recombinant protein. pass
Western Blotting (Recombinant protein) Antibody concentration: 1 ug/ml. Specific band of ~35.9 kDa using immunogen protein lysate. pass
Western Blotting (Transfected lysate) Antibody concentration: 0.1 ~ 5 ug/ml. Specific band of ~55.8 kDa using AKT3 transfected 293T lysate. pass