Certificate Of Analysis
Product Name Monoclonal Anti-AKT3 antibody produced in mouse,
clone 6E10, purified immunoglobulin, buffered aqueous solution
Product Number WH0010000M2
Product Brand SIGMA
 
TEST SPECIFICATION LOT 06188-6E10 RESULTS
Concentration 1 mg/ml
Isotype IgG2
Western Blotting (Cell lysate) Antibody concentration: 1 ~ 5 ug/ml. Specific band of ~56 kDa using MCF-7 lysate. pass
Immunofluorescence (IF) Antibody concentration: 10 ug/ml using MCF-7 cell. pass
Sandwich ELISA Detection limit for recombinant GST tagged AKT3 is approximately 1ng/ml. pass
ELISA (Recombinant protein) Antibody concentration: 1 ug/ml using recombinant protein. pass
Western Blotting (Recombinant protein) Antibody concentration: 1 ug/ml. Specific band of ~36 kDa using immunogen protein lysate. pass