Specification Sheet
Product Name HEPES,
BioUltra, for molecular biology, ≥99.5% (T)
Product Number 54457
Product Brand SIGMA
CAS Number 7365-45-9
Molecular Weight 238.30
 
TEST SPECIFICATION
APPEARANCE (COLOR) Colorless or White
APPEARANCE (FORM) Powder to Crystals
TITRATION (T) NAOH 0.1M ≥ 99.5 %
THIN LAYER CHROMATOGR. CORRESPONDS TO STANDARD
LOSS ON DRYING ≤ 0.5 %
SULFATED ASH ≤ 0.1 %
CARBON CONTENT 40.32 % (THEORY)
HYDROGEN CONTENT 7.61 % (THEORY)
NITROGEN CONTENT 11.76 % (THEORY)
INFRARED SPECTRUM CONFORMS TO STRUCTURE
METAL TRACE ANALYSIS (ICP) CORRESPONDS TO REQUIREMENTS
ALUMINIUM (ICP) ≤ 5 mg/kg
BARIUM (ICP) ≤ 5 mg/kg
BISMUTH (ICP) ≤ 5 mg/kg
CALCIUM (ICP) ≤ 10 mg/kg
CADMIUM (ICP) ≤ 5 mg/kg
COBALT (ICP) ≤ 5 mg/kg
CHROMIUM (ICP) ≤ 5 mg/kg
COPPER (ICP) ≤ 5 mg/kg
IRON (ICP) ≤ 5 mg/kg
POTASSIUM (ICP) ≤ 50 mg/kg
LITHIUM (ICP) ≤ 5 mg/kg
MAGNESIUM (ICP) ≤ 5 mg/kg
MANGANESE (ICP) ≤ 5 mg/kg
MOLYBDENUM (ICP) ≤ 5 mg/kg
SODIUM (ICP) ≤ 50 mg/kg
NICKEL (ICP) ≤ 5 mg/kg
LEAD (ICP) ≤ 5 mg/kg
STRONTIUM (ICP) ≤ 5 mg/kg
ZINC (ICP) ≤ 5 mg/kg
ARSENIC TRACES (MHS-AAS) ≤ 0.1 mg/kg
CHLORIDE (CL) ≤ 50 mg/kg
SULFATE (SO4) ≤ 50 mg/kg
EXTRANEOUS ACTIVITIES DNAses, RNAses, Proteases, Phosphatases Not Detectable
SOLUBILITY (METHOD) 7.149 G IN 30 ML H2O (1M)
APPEARANCE (SOLUTION) CLEAR COLORLESS
PH (SOLUTION) 5.0 - 6.5
RESIDUE (FILTER TEST) NO RESIDUE
UV - ABS. AT 260 NM ≤ 0.04
UV - ABS. AT 280 NM ≤ 0.04
CUT-OFF 230.0 - 250.0 nm
RECOMMENDED RETEST PERIOD 54 MONTHS