Specification Sheet
Product Name Trizma® base,
BioUltra, for molecular biology, ≥99.8% (T)
Product Number 93362
Product Brand SIGMA
CAS Number 77-86-1
Molecular Weight 121.14
 
TEST SPECIFICATION
APPEARANCE (COLOR) Colorless or White
APPEARANCE (FORM) Powder or Crystals
TITRATION (T) HCL 1M 99.8 - 100.5 %
MELTING POINT 168 - 172 C
LOSS ON DRYING ≤ 0.5 %
SULFATED ASH ≤ 0.01 %
INFRARED SPECTRUM CONFORMS TO STRUCTURE
LUMINESCENCE CORRESPONDS TO REQUIREMENTS
METAL TRACE ANALYSIS (ICP) CORRESPONDS TO REQUIREMENTS
ALUMINIUM (ICP) ≤ 5 mg/kg
BARIUM (ICP) ≤ 5 mg/kg
BISMUTH (ICP) ≤ 5 mg/kg
CALCIUM (ICP) ≤ 10 mg/kg
CADMIUM (ICP) ≤ 5 mg/kg
COBALT (ICP) ≤ 5 mg/kg
CHROMIUM (ICP) ≤ 5 mg/kg
COPPER (ICP) ≤ 5 mg/kg
IRON (ICP) ≤ 5 mg/kg
POTASSIUM (ICP) ≤ 50 mg/kg
LITHIUM (ICP) ≤ 5 mg/kg
MAGNESIUM (ICP) ≤ 5 mg/kg
MANGANESE (ICP) ≤ 5 mg/kg
MOLYBDENUM (ICP) ≤ 5 mg/kg
SODIUM (ICP) ≤ 50 mg/kg
NICKEL (ICP) ≤ 5 mg/kg
LEAD (ICP) ≤ 5 mg/kg
STRONTIUM (ICP) ≤ 5 mg/kg
ZINC (ICP) ≤ 5 mg/kg
ARSENIC TRACES (MHS-AAS) ≤ 0.1 mg/kg
CHLORIDE (CL) ≤ 20 mg/kg
SULFATE (SO4) ≤ 5 mg/kg
EXTRANEOUS ACTIVITIES DNAses, RNAses, Proteases, Phosphatases Not Detectable
SOLUBILITY (METHOD) 4M IN WATER
APPEARANCE (SOLUTION) CLEAR COLORLESS
PH (SOLUTION) 10.5 - 12.0
RESIDUE (FILTER TEST) NO RESIDUE
UV - ABS. AT 260 NM ≤ 0.10
UV - ABS. AT 280 NM ≤ 0.08
RECOMMENDED RETEST PERIOD 72 MONTHS