Specification Sheet
Product Name AKT3, active human,
buffered aqueous glycerol solution, protein apparent mol wt ~83 kDa, ≥85% (SDS-PAGE), recombinant, expressed in E. coli
Product Number A9104
Product Brand SIGMA
 
TEST SPECIFICATION
PROTEIN BY BRADFORD REPORT RESULT
ENZYMATIC ACTIVITY 300 - 3,000 UNITS/MG PROTEIN
UNIT DEFINITION ONE UNIT WILL INCORPORATE 1NANOMOLE OF PHOSPHATE INTO THE AKT/SGK SUBSTRATE PEPTIDE (RPRAATF) PER MINUTE AT PH7.2 AT 30DEGC USING A FINAL CONCENTRATION OF 50UM (32P)ATP.
PURITY BY SDS-PAGE NLT 85%
DOCUMENT # A9104/05/16/05/1