Specification Sheet
Product Name FGF-ACIDIC rat,
recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)
Product Number SRP3303
Product Brand SIGMA
Source recombinant, expressed in E. coli
Molecular Weight 15.9 kDa
Storage -20°C
Reconstitution Centrifuge the vial prior to opening. Reconstitute in 5 mM Sodium Phosphate, pH 7.2 to a concentration of >=0.5 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Appearance lyophilized powder
Color white to off-white
Purity by HPLC >=95%
Purity by SDS-PAGE >=95%
Endotoxin Level <= 1 EU/ug
Biological Activity Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is >= 0.1 ng/ml, in the presence of 10ug/ml heparin, corresponding to a specific activity of >= 1 x 10^7 units/mg.