Specification Sheet
Product Name TFPI human,
recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Product Number SRP6458
Product Brand SIGMA
Appearance Report Result
Purity by SDS-PAGE ≥95%
Endotoxin <1 EU/µg by LAL method
Biological Activity Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca - RPKPVE - Nval - WRK (Dnp) - NH2. The IC50 value is < 0.35 nM, as measured in 100 µl reaction mixture containing 1.25 ng trypsin, 10 µM substrate, 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35 (w/v), pH 7.5