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Biotechnology and bioengineering

Selective separation of trypsin from pancreatin using bioaffinity in reverse micellar system composed of a nonionic surfactant.


PMID 10099303

Abstract

Selective separation of trypsin from a mixture involving many kinds of contaminating proteins, i.e., pancreatin, was achieved using trypsin inhibitor immobilized in the reverse micelles, which were composed of a nonionic surfactant, tetra-oxyethylene monodecylether. To determine the efficient operations throughout the whole separation process we examined the operating conditions, which affect the immobilization efficiency of trypsin inhibitor and also the forward and backward extractions of trypsin. Fifty percent of the recovery of trypsin from pancreatin was realized with no loss of activity of the recovered trypsin.

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86694
Tetraethylene glycol monododecyl ether, BioXtra, ≥98.0% (GC)
C20H42O5