EMAIL THIS PAGE TO A FRIEND

Sheng wu gong cheng xue bao = Chinese journal of biotechnology

[Cloning and expression of a synthetic gene encoding magainin-melittin hybrid peptide in Escherichia coli and studies on its antibacterial activity].


PMID 11411233

Abstract

A hybrid peptide gene was designed and synthesized. Its encoding peptide is constructed from residues 3-14 of magainin and residues 1-13 of melittin. The MA-E gene was cloned into plasmids pUC18 and pBV220. By DNA sequencing, the whole sequences of this gene is confirmed to be correct. The recombinant plasmid pBMA-E was expressed in E. coli DH5 alpha. A gene product band can be seen with Tricine-SDS-PAGE. The MA-E hybrid peptide was purified by immobilized metal affinity chromatography. Bioactivity assay was carried out in liquid turbidity method. The bactericide value to E. coli K12D31 is 0.182.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

M7152
Magainin I, ≥97% (HPLC)
C112H177N29O28S