EMAIL THIS PAGE TO A FRIEND

Journal of bacteriology

The mere lack of rT modification in initiator tRNA does not facilitate formylation-independent initiation in Escherichia coli.


PMID 11717300

Abstract

Formylation of initiator methionyl-tRNA is essential for normal growth of eubacteria. However, under special conditions, it has been possible to initiate protein synthesis with unformylated initiator tRNA even in eubacteria. Earlier studies suggested that the lack of ribothymidine (rT) modification in initiator tRNA may facilitate initiation in the absence of formylation. In this report we show, by using trmA strains of Escherichia coli (defective for rT modification) and a sensitive in vivo initiation assay system, that the lack of rT modification in the initiators is not sufficient to effect formylation-independent initiation of protein synthesis.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

M8905
5-Methyluridine, powder
C10H14N2O6
535893
5-Methyluridine, 97%
C10H14N2O6
F3377
N-Formyl-L-methionine
C6H11NO3S