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[Effect of CrCl3 on the in vitro production of flavonoids in a culture of Ononis arvensis L].


PMID 11910742

Abstract

The paper investigated the effect of the abiotic elicitor chromium trichloride on the production of flavonoids in the callus and suspension cultures of Ononis arvensis L. after 12; 24; 48; 72, and 168-hour administration. The tested elicitor positively influenced the formation of flavonoids. A statistically significant increase in the flavonoid production in the callus culture was recorded with the use of a chromium trichloride solution in the concentration c1 (6.32 x 10(-4) mol/l) after 24; 48; 72, and 168 hours, in the concentration c2 (6.32 x 10(-6) mol/l) after 24; 48, and 72 hours, and in the concentration c3 (6.32 x 10(-8) mol/l) after 12; 24, and 48 hours. In the suspension culture, the flavonoid production was significantly increased by the concentration c1 (6.32 x 10(-4) mol/l) after 12 and 72 hours, the concentration c2 (6.32 x 10(-6) mol/l) after 12; 24; 48, and 72 hours, and the concentration c3 (6.32 x 10(-8) mol/l) after 12; 24; 48, and 72 hours. The maximal increase in the flavonoid production by 98% took place in the callus culture after elicitation with a chromium trichloride in the concentration c3 (6.32 x 10(-8) mol/l) after 48 hours of administration, and in the suspension culture after elicitation with CrCl3 in the concentration c2 (6.32 x 10(-6) mol/l) after 12 hours by 100%.