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Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Expression of Brain-derived Neurotrophic Factor (BDNF) Gene in Rat Myoblast Cells.


PMID 12142916

Abstract

Brain-derived neurotrophic factor (BDNF) gene was cloned into retrovirous vector plasmid pLNCX to form the pLNC/BDNF. After packaging in PA317 cell line, the infectious particles were used to infect rat myoblast cell line L-6TG. After selection with G418, the L-6TG/BDNF cells were collected. Results from Southern blot show that bdnf gene has successfully integrated into the L-6TG genomic DNA. The expression of bdnf gene has been proved by Northern blot and Dot blot. The content of BDNF in the culture medium of L-6TG/BDNF was about 25 ng.10(-6) cells per 24 h per ml.

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PA317, 89032007