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Archives of histology and cytology

Preparation of human chromosomes for high resolution scanning electron microscopy.


PMID 1290676

Abstract

The addition of ethidium bromide during the last 2.5-3 h of lymphocyte culturing restricted chromosome contraction and preserved the banding structure in scanning electron microscopy. Treatment of the chromosomes with trypsin and use of impregnation with osmium tetroxide and thiocarbohydrazide resulted in a structural preservation of high resolution quality.

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223220
Thiocarbohydrazide, 98%
CH6N4S