EMAIL THIS PAGE TO A FRIEND

Journal of pharmaceutical and biomedical analysis

Analysis of fluorophore-labelled hyaluronan and chondroitin sulfate disaccharides in biological samples.


PMID 15019056

Abstract

In this report, we describe a polyacrylamide gel electrophoresis for the analysis of fluorophore-labelled hyaluronan (HA) and chondroitin sulfate (CS) Delta-disaccharides. The method utilizes derivatization of reducing ends of hyaluronan and the variously sulfated chondroitin sulfate Delta-disaccharides with 2-aminoacridone (AMAC), followed by electrophoresis in Tris-HCl buffer (pH 8.8), and polyacrylamide gel (T 25%/C 3.75%). The method was applied to the analysis of GAGs secreted into the culture medium of human aortic smooth muscle cells and the obtained results were compared with those analysed by fluorophore assisted carbohydrate electrophoresis (FACE). The described method is a useful and sensitive tool for the rapid monitoring of GAGs in high number of biologic samples.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

06627
2-Aminoacridone, BioReagent, suitable for fluorescence, ≥98.0% (HPLC)
C13H10N2O