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Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

[Determination of protopine and isocorydine in root of Dactylicapnos scandens by HPLC].


PMID 15631083

Abstract

To establish a HPLC method for determination of protopine and isocorydine in root of Dactylicapnos scandens. The separation was performed in a PHENOMENEX-C18 column with a mobile phase of methanol-0.2% phosphoric acid (adjusted to pH 7.0 with triethylamine)(50:50), The detection wavelength was at 254 nm and the flow rate was 0.8 mL x min(-1). The average recovery of Protopine and Isocorydine was 97.9%, 98.6% respectively, and RSD 1.3%, 1.4%. This method is accurate, simple and reliable. It can be used for quality control of D. scandens.