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Guang pu xue yu guang pu fen xi = Guang pu

[Study on the reaction mechanism of chloroacetanilide herbicides with urease using fluorescence spectrum and high-performance liquid chromatography].


PMID 16013334

Abstract

The relationship between excess thermodynamic function and binding to urease of four chloroacetanilide herbicides was studied using high-performance liquid chromatography and fluorescence spectrum. The linear relationship between the composition of mobile phase of RP-HPLC and the capacity factor of chloroacetanilide herbicides has been obtained. The excess thermodynamic enthalpy (deltaH* ) of acetochlor, pretilachlor, butachlor and metolachlor was determined, and the binding constant K and the number of binding sites with urease were calculated. The relationship between excess thermodynamic function and the binding constant K was suggested.

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