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Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Pseudouridine detection improvement by derivatization with methyl vinyl sulfone and capillary HPLC-mass spectrometry.


PMID 16029966

Abstract

A method is presented for improved detection of pseudouridine in nucleoside mixtures based on the specific derivatization with methyl vinyl sulfone followed by analysis by capillary HPLC-mass spectrometry. Reaction conditions were optimized in order to obtain the best yield and specificity. The method was successfully applied to different nucleoside mixtures.

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247197
Methyl vinyl sulfone, ≥97%
C3H6O2S