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Biotechnology and bioengineering

Prevention of thermal induced aggregation of cytochrome c at isoelectric pH values by polyanions.


PMID 16273554

Abstract

Differential scanning calorimetry, viscometry, optical and CD spectroscopy were used to characterize the influence of two polyanions, poly(vinylsulfate) (PVS), and poly(4-styrene-sulfonate) (PSS) on thermal transition reversibility of ferricytochrome c at or near isoelectric pH. In these conditions, both PVS and PSS enhance the thermal transition reversibility of cytochrome c by preventing the aggregation of denatured protein molecules. Data indicate that the polyanions are in complex with cytochrome c that is stabilized by synergistic effect of Coulombic and non-Coulombic interactions.

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271969
Poly(vinyl sulfate) potassium salt, average Mw ~170,000