EMAIL THIS PAGE TO A FRIEND

Journal of chromatography. A

Enantioselective high-performance liquid chromatographic separation of N-methyloxycarbonyl unsaturated amino acids on macrocyclic glycopeptide stationary phases.


PMID 16443231

Abstract

This paper describes the enantiomeric resolution of a series of unsaturated N-methyloxycarbonyl-alpha-H-alpha-amino acids (N-MOC-alpha-amino acids) on macrocyclic glycopeptide stationary phases by means of high-performance liquid chromatography (HPLC). Three types of glycopeptide phases, i.e. Chirobiotic T, V and R, were evaluated in both reversed-phase (RP) and polar ionic mode (PIM). The best results in terms of enantioselectivity and resolution were obtained on Chirobiotic R phase, with the PIM mobile phase giving the highest resolution per min. Investigation of the pH of the reversed-phase mobile phase in the pH range 4.1-5.9 showed little effect on enantioselectivity. The method was applied for monitoring the conversion and product enantiomeric excess of an enzymatic hydrolysis reaction using N-MOC-alpha-H-alpha-amino acid esters as substrate.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

13019AST Astec® CHIROBIOTIC® R Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2.1 mm
13020AST Astec® CHIROBIOTIC® R Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 2.1 mm
13010AST Astec® CHIROBIOTIC® R Chiral HPLC Column, 5 μm particle size, L × I.D. 10 cm × 3 mm
13022AST Astec® CHIROBIOTIC® R Chiral HPLC Column, 5 μm particle size, L × I.D. 10 cm × 4.6 mm
13023AST Astec® CHIROBIOTIC® R Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
13024AST Astec® CHIROBIOTIC® R Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
12018AST Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 10 cm × 2.1 mm
12019AST Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2.1 mm
12020AST Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 2.1 mm
12010AST Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 10 cm × 3 mm
12021AST Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
12022AST Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 10 cm × 4.6 mm
12023AST Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
12024AST Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
12034AST Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 10 mm
11018AST Astec® CHIROBIOTIC® V Chiral HPLC Column, 5 μm particle size, L × I.D. 10 cm × 2.1 mm
11019AST Astec® CHIROBIOTIC® V Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2.1 mm
11020AST Astec® CHIROBIOTIC® V Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 2.1 mm
11010AST Astec® CHIROBIOTIC® V Chiral HPLC Column, 5 μm particle size, L × I.D. 10 cm × 3 mm
11022AST Astec® CHIROBIOTIC® V Chiral HPLC Column, 5 μm particle size, L × I.D. 10 cm × 4.6 mm
11023AST Astec® CHIROBIOTIC® V Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
11024AST Astec® CHIROBIOTIC® V Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
11034AST Astec® CHIROBIOTIC® V Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 10 mm
11044AST Astec® CHIROBIOTIC® V Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 21.2 mm