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Applied and environmental microbiology

Purification and characterization of two extracellular alkaline phosphatases from a psychrophilic arthrobacter isolate.


PMID 16535659

Abstract

Two extracellular, heat-labile alkaline phosphatases were purified from a psychrophilic Arthrobacter isolate, D10. The enzymes were active over different pH ranges, used distinct substrates, and had different kinetic properties. Each enzyme reacted specifically to its own antibody during immunoblot analysis. One had both monophosphatase and diesterase activities.

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R0503 Reactive Red 120−Agarose, saline suspension, Type 3000-CL