EMAIL THIS PAGE TO A FRIEND

Analytical and bioanalytical chemistry

Cu2+ and Cu+ bathocuproine disulfonate complexes promote the oxidation of the ROS-detecting compound dichlorofluorescin (DCFH).


PMID 16791578

Abstract

The water-soluble Cu+ chelator bathocuproine disulfonate (BCS) is widely used to quantify Cu+ or detect Cu+ formation in Cu2+-initiated oxidation reactions. The dichlorofluorescin (DCFH) assay is commonly used to monitor free radical reactions, reactive oxygen species (ROS), or reactive nitrogen species (RNS). Upon oxidation the non-fluorescent DCFH is converted into the fluorescent compound dichlorofluorescein (DCF). In the present communication we show that the Cu+ reagent BCS strongly facilitated the oxidation of DCFH in the presence of Cu2+ or Cu+. In contrast, 2,2'-dipyridyl (DP), which is also a Cu+-complexing reagent, but not as well known and therefore not as commonly used as BCS, did not cause any oxidative modification of DCFH in the presence of Cu2+ or Cu+. We therefore recommend that DP should be used instead of BCS to complex Cu+ in reactions which are initiated by Cu2+ and when ROS/RNS are analyzed by the DCFH oxidation assay.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

B1125
Bathocuproinedisulfonic acid disodium salt, for spectrophotometric det. of Cu, Fe
C26H18N2Na2O6S2