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Bulletin of experimental biology and medicine

Express method for detection of Amanita phalloides amanitine toxins.


PMID 16929979

Abstract

Spectral studies of the interaction between amanitine and ethidium bromide fluorophore showed the appearance of a new intensive fluorescence band after addition of amanitine to ethidium bromide solution, caused by the formation of a charge-transfer complex. The new fluorescence band is located in a shorter wave region of the spectrum compared to ethidium bromide fluorescence band. Based on the results, a rapid fluorescent method for detection of amanitines was developed.

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A1304
β-Amanitin from Amanita phalloides, ~90% (HPLC)
C39H53N9O15S