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Wei sheng yan jiu = Journal of hygiene research

[Study of dicyclohexyl phthalate on preparation and characterization of artificial antigen].


PMID 17086698

Abstract

To study the preparation and characterization of hapten and artificial antigen of dicyclohexyl phthalate. A dicyclohexyl phthalate hapten (4-amino dicyclohexyl phthalate) was synthesized by introducing amino as a substituent on the aromatic ring and retaining the ester group, and characterized by 1HNMR, IR and UV. The hapten was conjugated to BSA via amino diazotization linkage. Lambda1 = 214nm, lambda2 = 256nm for the UV of dicyclohexyl 4-nitrophthalate and lambda1 = 226nm, lambda2 = 288nm for the UV of dicyclohexyl 4-aminophthalate. Artificial antigen was prepared and tested by fluorescence, and lambda(ex) = 307nm, lambda(em) = 468nm, and the approximate molar ratio of dicyclohexyl 4-aminophthalate to BSA was 19. The product was used as an immunogen, demonstrating that it is suitable for polyclonal antibody production. It is a good method for preparation of artificial antigen of dicyclohexyl phthalate by introducing amino as a substituent on the aromatic ring and retaining the ester group. It was suggested that could supply excellent immune antigen for further preparation of antibody and immunoassay to dicyclohexyl phthalate.

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