EMAIL THIS PAGE TO A FRIEND

Luminescence : the journal of biological and chemical luminescence

Chemiluminescence assay for tetrahydrobiopterin based on the generation of hydrogen peroxide using isoluminol-microperoxidase in the presence of 1-methoxy PMS.


PMID 17285567

Abstract

We developed a novel highly sensitive chemiluminescence (CL) method for BH(4). The principle of the proposed method is based on active oxygen formation induced by 1-methoxy-5-methyl phenazinium methyl sulphate (1-methoxy PMS) in the presence of dissolved oxygen. Furthermore, active oxygen is determined by a CL assay involving the luminol reaction with microperoxidase. In this report, we examined the mechanism of formation and identified the reactive oxygen species derived from BH(4) employing 1-methoxy PMS. Additionally, optimum conditions for the CL assay of BH(4) were established.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

M8640
1-Methoxy-5-methylphenazinium methyl sulfate, ≥95%
C15H16N2O5S
A8264
4-Aminophthalhydrazide
C8H7N3O2