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Journal of chromatography. A

Effect on separation of injecting samples in a solvent different from the mobile phase.


PMID 17331517

Abstract

The separation of compounds possessing low solubility in the mobile phase could be improved by applying stronger solvents for dissolving the feed. A model system has been investigated with ethanol-water as the mobile phase, Chirobiotic T as the stationary phase and D,L-threonine as the sample dissolved in pure water. The adsorption isotherms of D,L-threonine were determined as a function of the water content by means of a peak fitting method. Optimal injection conditions of elution chromatography were determined by process simulation. Finally, the optima obtained were verified with experimental data.