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Wei sheng yan jiu = Journal of hygiene research

[Simultaneous determination of nine beta2-agonists residues in urines by ultra performance liquid chromatography-electrospray tandem mass spectrometry].


PMID 17953220

Abstract

To study the determination method of nine beta2-agonists residues in urine. Urine samples were duconjugated with beta-glucuronidase/arylsulfatase enzyme in acetate buffer and deproteinized by perchloric acid, and then adjusted pH4.0. Sample concentration and purification were performed by Oasis HLB, Oasis MCX. The separation was performed on a Waters ACQUITY UPLCTM BEH C18 column (100mm x 2.1mm i.d., 1.7microm) with gradient elution using methanol and water (containing 0.1% formic acid) at a flow rate of 0.3ml/min. The limits of detection (LOD) of the method were from 0.002 to 0.025ng/ml and the limits of quantification (LOQ) ranged from 0.007 to 0.08ng/ml. Average recoveries for nine beta2-agonists at the spiking levels of 0.1, 0.5 and 2ng/ml ranged from 77.4% to 101.7% with relative standard deviations between 3.4% and 18% . The method can be used to determine the residues of nine beta2-agonists in urines.

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