EMAIL THIS PAGE TO A FRIEND

Journal of the American Society for Mass Spectrometry

Counting sulfhydryls and disulfide bonds in peptides and proteins using mercurial ions as an MS-tag.


PMID 18524619

Abstract

Organic mercurial compounds are the most specific and sensitive reagents for reaction with the sulfhydryl groups (SHs) in peptides and proteins because of the strong mercury-sulfur affinity. Using the monofunctional organic mercury ion RHg(+) as a mass spectrometry (MS)-tag has the advantages of reacting with one sulfhydryl group, offering definite mass shift, and especially stable and characteristic nonradioactive isotopic distribution. Mass spectrometric analysis of derivatized sulfhydryls in peptides/proteins is thus an alternative for precisely counting the number of sulfhydryl groups and disulfide bonds (SS). Here the tags used include monomethylmercury chloride, monoethylmercury chloride, and 4-(hydroxymercuri) benzoic acid. The feasibility of this strategy is demonstrated using HPLC/ESI-MS to count SHs and SS in model peptides/proteins, i.e., glutathione, phytochelatins, lysozyme and beta-lactoglobulin, which contain increasing SHs and various SS linkages.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

55540
4-(Hydroxymercuri)benzoic acid sodium salt, ≥95.0% (Hg)
C7H5HgNaO3