EMAIL THIS PAGE TO A FRIEND

Proceedings of the National Academy of Sciences of the United States of America

A quantitative atlas of mitotic phosphorylation.


PMID 18669648

Abstract

The eukaryotic cell division cycle is characterized by a sequence of orderly and highly regulated events resulting in the duplication and separation of all cellular material into two newly formed daughter cells. Protein phosphorylation by cyclin-dependent kinases (CDKs) drives this cycle. To gain further insight into how phosphorylation regulates the cell cycle, we sought to identify proteins whose phosphorylation is cell cycle regulated. Using stable isotope labeling along with a two-step strategy for phosphopeptide enrichment and high mass accuracy mass spectrometry, we examined protein phosphorylation in a human cell line arrested in the G(1) and mitotic phases of the cell cycle. We report the identification of >14,000 different phosphorylation events, more than half of which, to our knowledge, have not been described in the literature, along with relative quantitative data for the majority of these sites. We observed >1,000 proteins with increased phosphorylation in mitosis including many known cell cycle regulators. The majority of sites on regulated phosphopeptides lie in [S/T]P motifs, the minimum required sequence for CDKs, suggesting that many of the proteins may be CDK substrates. Analysis of non-proline site-containing phosphopeptides identified two unique motifs that suggest there are at least two undiscovered mitotic kinases.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

P7634 3-Phosphoglyceric Phosphokinase from baker's yeast (S. cerevisiae), ammonium sulfate suspension, ≥1000 units/mg protein
N8661 5′-Nucleotidase from Crotalus atrox venom, lyophilized powder, ≥200 units/mg protein
N1665 5′-Nucleotidase human, recombinant, expressed in CHO cells, vial of 6-12 μg
A6986 Acetyl-CoA Carboxylase 1 human, recombinant, expressed in Sf9 cells
A6861 Acetyl-CoA carboxylase 2 human, recombinant, expressed in Sf9 cells
A3352 Acyl-coenzyme A Synthetase from Pseudomonas sp., ≥2 units/mg protein
A7510 Adenosine 5′-Triphosphatase from porcine cerebral cortex, lyophilized powder, ≥0.3 units/mg protein, pH 7.8
A8811 Aldolase from rabbit muscle, ammonium sulfate suspension, 10-20 units/mg protein
A2714 Aldolase from rabbit muscle, lyophilized powder, ≥8.0 units/mg protein
A9329 Aldolase from spinach, lyophilized powder, 0.3-1.0 units/mg protein
C7886 Creatine Phosphokinase from bovine heart, Type III, salt-free, lyophilized powder, ≥30 units/mg protein
C3755 Creatine Phosphokinase from rabbit muscle, Type I, salt-free, lyophilized powder, ≥150 units/mg protein
D9380 DNA Polymerase I from Escherichia coli lysogenic for NM 964, buffered aqueous glycerol solution
E6126 Enolase from baker's yeast (S. cerevisiae), lyophilized powder, ≥50 units/mg protein
F0137 Fructose-6-phosphate Kinase from Bacillus stearothermophilus, Type VII, lyophilized powder, ≥50 units/mg protein
G5262 GAPDH, standard for protein electrophoresis
G6378 Glucose-6-phosphate Dehydrogenase from baker's yeast (S. cerevisiae), Type XV, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)
G7877 Glucose-6-phosphate Dehydrogenase from baker's yeast (S. cerevisiae), Type VII, ammonium sulfate suspension, ≥200 units/mg protein
G4134 Glucose-6-phosphate Dehydrogenase from baker's yeast (S. cerevisiae), Type IX, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)
G5885 Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides, lyophilized powder, 550-1,100 units/mg protein (biuret)
G8404 Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides, recombinant, expressed in E. coli, ammonium sulfate suspension, 550-1,100 units/mg protein (biuret)
G5760 Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides, Type XXIII, ammonium sulfate suspension, 550-1,100 units/mg protein (biuret), ≥2.0 mg/mL Biuret
G8529 Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides, recombinant, expressed in E. coli, lyophilized powder, 550-1,100 units/mg protein (biuret)
G2921
Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides, BioUltra, recombinant, expressed in E. coli, ammonium sulfate suspension, ≥95% (SDS-PAGE), ≥550 units/mg protein (biuret)
G8289 Glucose-6-phosphate dehydrogenase from torula yeast, ammonium sulfate suspension, 300-600 units/mg protein (biuret)
G0389 Glyceraldehyde-3-phosphate Dehydrogenase Agarose from baker's yeast (S. cerevisiae), lyophilized powder, 50-150 units/g dry agarose, pH 7.6, 30 °C
G5537 Glyceraldehyde-3-phosphate Dehydrogenase from baker's yeast (S. cerevisiae), lyophilized powder, 70-140 units/mg protein
G9263 Glyceraldehyde-3-phosphate Dehydrogenase from chicken muscle, lyophilized powder, ≥40 units/mg protein
G6019 Glyceraldehyde-3-phosphate Dehydrogenase from human erythrocytes, lyophilized powder, 50-150 units/mg protein
G2267 Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle, lyophilized powder, ≥75 units/mg protein