EMAIL THIS PAGE TO A FRIEND

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience

Lysine induces lipid and protein damage and decreases reduced glutathione concentrations in brain of young rats.


PMID 18691648

Abstract

The present work investigated the in vitro effects of lysine on important parameters of oxidative stress in cerebral cortex of young rats. Our results show that lysine significantly induced lipid peroxidation, as determined by increase of thiobarbituric acid-reactive substances and chemiluminescence levels, as well as protein oxidative damage since carbonyl formation and sulfhydryl oxidation were enhanced by this amino acid. Furthermore, the addition of free radical scavengers significantly prevented lysine-induced lipid oxidative damage, suggesting that free radicals were involved in this effect. Lysine also significantly diminished glutathione levels in cortical supernatants, decreasing, therefore, the major brain antioxidant defense. Finally, lysine markedly oxidized a glutathione commercial solution in a medium devoid of brain supernatants, indicating that it behaved as a direct acting oxidant. The present data indicate that lysine induces oxidative stress in cerebral cortex of young rats. Therefore, it is presumed that this pathomechanism may be involved at least in part in the neurological damage found in patients affected by disorders with hyperlysinemia.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

481718
Iron(0) pentacarbonyl, >99.99% trace metals basis
C5FeO5
195731
Iron(0) pentacarbonyl
C5FeO5