Toxicology in vitro : an international journal published in association with BIBRA

The effect of lead on intracellular Ca(2+) in mouse lymphocytes.

PMID 18789383


Lead exposure is one of major public health problems. The aim of this study was to examine in vitro the effect of lead on lymphocyte intracellular Ca(2+) ([Ca(2+)]i). Lymphocytes were separated from mice spleen and treated with three doses of PbCl(2) (1, 10 and 100 micromol/L). [Ca(2+)]i was measured in a Thermo Labsystems Fluoroskan Ascent after loading the cells with Fura2-AM probe. The lymphocyte [Ca(2+)]i in 10 and 100 micromol/L PbCl(2) groups increased to the highest level after 10 min of lead exposure (P<0.05). After 1h of lead exposure, however, lymphocyte [Ca(2+)]i levels were not statistically different from that in control group cells (P>0.05). Removal of Ca(2+) from external solution did not significantly affect the PbCl(2)-induced lymphocyte [Ca(2+)]i. PbCl(2) increased the [Ca(2+)]i under both normal Ca(2+) and Ca(2+)-free conditions. With pretreatment of calmodulin (CaM) antagonist W7, lymphocyte [Ca(2+)]i levels were still high, but [Ca(2+)]i levels were not as high as those in the absence of W7. When the lymphocytes were exposed to PbCl(2) ranging from 1 to 100 micromol/L, the lymphocyte [Ca(2+)]i level was increased, but the increase appeared reversible(.) CaM may play a role in the process of the effect of lead on the lymphocyte [Ca(2+)]i.