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Analytical biochemistry

Estimation of peptide concentration by a modified bicinchoninic acid assay.


PMID 19539599

Abstract

Although biuret based protein assays are theoretically applicable to peptide measurement, there is a high level of interpeptide variation, determined largely by peptide hydrophobicity. This variation in peptide reactivity can be significantly reduced by heat-denaturation of peptides at 95 degrees C for 5 min in the presence of 0.1 M NaOH containing 1% (w/v) SDS, prior to incubation for 30 min at 37 degrees C in BCA standard working reagent. This modification to the standard bicinchoninic acid (BCA) assay protocol allows for an accurate, rapid, and economical estimation of the peptide concentration within an unknown sample.

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14335
2,2′-Biquinoline-4,4′-dicarboxylic acid, ≥90% (TLC)
C20H12N2O4