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Methods in molecular biology (Clifton, N.J.)

Expression of single-chain Fv fragments in E. coli cytoplasm.


PMID 19554299

Abstract

The most frequently used approach to produce single-chain Fv fragments (scFv) and Fab in Escherichia coli is to express them in the periplasm of the bacteria. We present here an alternative procedure that uses cytoplasmic expression of soluble active scFv. This can be accomplished by using either specially engineered E. coli strains or hyperstable scFvs.