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Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

[Hairy root culture optimization and aucubin medical composition production of Eucommia ulmoides].


PMID 19894530

Abstract

To introduce the hairy root of Eucommia ulmoides, and screen put the excellent roots. Hairy roots were obtained by infecting the different explants of E. ulmoides. Aucubin content was detected by HPLC. The best transformation condition was embryonic axis infected by twenty minutes bacillus water dip-dye and three-days co-culture. Liquid culture was better than solid culture, and advantageous to accumulating of biomass and medical contents of hairy root. The E1 was the excellent roots. Hairy root of E. ulmoides will be useful for the production of active components in aucubin.