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Analytical biochemistry

A visible dye-based staining method for DNA in polyacrylamide gels by ethyl violet.


PMID 20230772

Abstract

We describe a visible dye-based staining method for DNA in polyacrylamide gels using ethyl violet (EV). The novel method is a background-free, sensitive, economical, and simple procedure involving only staining and washing steps that can be completed within 30 min. As little as 0.8-1.6 ng of phiX174 DNA/HaeIII can be detected by EV, which is about eightfold more sensitive than Nile blue (NB) stain and twofold less sensitive than ethidium bromide (EB) stain.

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228842
Ethyl Violet, cationic triarylmethane dye
C31H42ClN3