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Langmuir : the ACS journal of surfaces and colloids

Reduced bacterial deposition and attachment by quorum-sensing inhibitor 4-nitro-pyridine-N-oxide: the role of physicochemical effects.


PMID 20553026

Abstract

Surface-attached chemical groups that resist protein adhesion are commonly characterized as being hydrophilic, H-bond acceptors, non-H-bond donors, and electrically neutral. Quorum-sensing (QS) inhibitor 4-nitropyridine-N-oxide (4-NPO) that previously was found to decrease Pseudomonas aeruginosa biofilm formation possesses all of these characteristics, making this molecule an ideal antiadhesive compound. It was hypothesized that once 4-NPO adsorbs to either the solid surface or bacteria, resultant changes in the physical-chemical surface properties of the solid surface and bacteria will reduce the extent of bacterial adhesion. These physical-chemical effects take place prior to the commencement of already well-established QS biofilm-inhibition mechanisms. Bacterial adhesion experiments to silica conducted in quartz crystal microbalance with dissipation (QCM-D) and parallel plate flow cells demonstrated that 4-NPO reduces bacterial adhesion to silica-coated surfaces by the adsorption of 4-NPO to the silica surface as well to the outer membrane of both gram-negative P. aeruginosa PAO1 and gram-positive Staphylococcus aureus. 4-NPO effectively neutralizes both the bacterial and silica surface charge, and it is proposed that this neutralization of local surface charge heterogeneities by 4-NPO adsorption is the mechanism responsible for decelerating rates of bacterial deposition.

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346659
4-Nitropyridine N-oxide, 97%
C5H4N2O3