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Voprosy pitaniia

[Selection of the optimal method of extraction and determination of dye Sudan I-IV and Para Red by high-performance liquid chromatography].


PMID 20560487

Abstract

Since 2004 was important the problem of determination of non-food dyes as Sudan I-IV and Para Red. Therefore it was necessary to develop reliable methodologies or adapt existing analytical methods for monitoring the presence of such dyes in food. For this purpose, we have previously proposed a method for high-performance liquid chromatography with diode-array and mass detection. However this method showed the urgent need to clarify this important phase of the study as the sample preparation. In the present work was studied the possibility of solving this problem such solvents as ethanol, chloroform, acetone, conducted the selection of the optimum ratio of extraction agent-raw. The authors have also refined the metrological characteristics of the method.