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Bioscience, biotechnology, and biochemistry

Practical preparation of epilactose produced with cellobiose 2-epimerase from Ruminococcus albus NE1.


PMID 20699553

Abstract

A practical purification method for a non-digestible disaccharide, epilactose (4-O-beta-galactosyl-D-mannose), was established. Epilactose was synthesized from lactose with cellobiose 2-epimerase and purified by the following procedure: (i) removal of lactose by crystallization, (ii) hydrolysis of lactose by beta-galactosidase, (iii) digestion of monosaccharides by yeast, and (iv) column chromatography with Na-form cation exchange resin. Epilactose of 91.1% purity was recovered at 42.5% yield.

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1236801 Epilactose, United States Pharmacopeia (USP) Reference Standard
C12H22O11