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Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993)

[Screening of yeast-producers of alpha-L-rhamnosidase].


PMID 21381311

Abstract

Screening of producers of alpha-L-rhamnosidase among 331 yeast strains (representatives of 12 genera and 17 species) has been performed. A capacity to synthesize the enzyme was revealed in 45% of the studied strains with activity 0.01-0.6 un/ml. Complex enzymatic preparations of alpha-L-rhamnosidase were obtained from cultural liquid of two the most active producers by fractionation with ammonium sulfate (90% saturation). The influence of pH and temperature on enzymes activities and also specificity towards natural and synthetic substrates were studied.