EMAIL THIS PAGE TO A FRIEND

Wei sheng yan jiu = Journal of hygiene research

[Study of three methods on joint toxicity of diazinon, propoxur and bisphenol A on proliferation of mouse RAW264.7 cell].


PMID 21560307

Abstract

To investigate possible joint toxic effects of diazinon, propoxur and bisphenol A (BPA) on proliferation of RAW264.7 cells in vitro. Cytotoxicity was assessed by MTT assay. The median inhibiting concentration values (IC50) and 95% confidence interval (CI) of diazinon, propoxur and BPA individually and in mixture (mixed according to ratio of IC50) were established by weighted probit method. The types of toxic interaction of diazinon & BPA and propoxur & BPA were assessed by three methods commonly used for binary mixtures, which were Additional Index Method, Equivalent Effect Curve Method and Logistic Regression Method. After 24-hour expoxure, the IC50 and 95% CI of diazinon, propoxur and BPA to RAW264.7 cells were 194.1 microg/ml (173.4 microg/ml-217.4 microg/ml), 448.4 mg/L (358.2 microg/ml-573.2 microg/ml) , and 37.5 microg/ml (35.3 microg/ml-39.9 microg/ml), respectively. Those of mixtures of diazinon & BPA and propoxur & BPA were 168.8 microg/ml (160.1 microg/ml-178.2 microg/ml) and 253.4 microg/ml (236.0-273.0 microg/ml). In the interaction assessment, three methods all demonstrated an antagonistic action of diazinon & BPA and an addition action of propoxur & BPA. It is concluded that the interaction of diazinon & BPA on proliferation of RAW264.7 cells is antagonistic, and that of propoxur & BPA is additive.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

45644
Propoxur, PESTANAL®, analytical standard
C11H15NO3