EMAIL THIS PAGE TO A FRIEND

Lab on a chip

High throughput label-free platform for statistical bio-molecular sensing.


PMID 21623438

Abstract

Sensors are crucial in many daily operations including security, environmental control, human diagnostics and patient monitoring. Screening and online monitoring require reliable and high-throughput sensing. We report on the demonstration of a high-throughput label-free sensor platform utilizing cantilever based sensors. These sensors have often been acclaimed to facilitate highly parallelized operation. Unfortunately, so far no concept has been presented which offers large datasets as well as easy liquid sample handling. We use optics and mechanics from a DVD player to handle liquid samples and to read-out cantilever deflection and resonant frequency. Also, surface roughness is measured. When combined with cantilever deflection the roughness is discovered to hold valuable additional information on specific and unspecific binding events. In a few minutes, 30 liquid samples can be analyzed in parallel, each by 24 cantilever-based sensors. The approach was used to detect the binding of streptavidin and antibodies.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

36605
2,6-Dichlorobenzamide, PESTANAL®, analytical standard
C7H5Cl2NO