EMAIL THIS PAGE TO A FRIEND

Science (New York, N.Y.)

Mutagenic processing of ribonucleotides in DNA by yeast topoisomerase I.


PMID 21700875

Abstract

The ribonuclease (RNase) H class of enzymes degrades the RNA component of RNA:DNA hybrids and is important in nucleic acid metabolism. RNase H2 is specialized to remove single ribonucleotides [ribonucleoside monophosphates (rNMPs)] from duplex DNA, and its absence in budding yeast has been associated with the accumulation of deletions within short tandem repeats. Here, we demonstrate that rNMP-associated deletion formation requires the activity of Top1, a topoisomerase that relaxes supercoils by reversibly nicking duplex DNA. The reported studies extend the role of Top1 to include the processing of rNMPs in genomic DNA into irreversible single-strand breaks, an activity that can have distinct mutagenic consequences and may be relevant to human disease.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

C1625
L-Canavanine, ≥98% (TLC), powder, from Canavalia ensiformis
C5H12N4O3