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Analytical biochemistry

Monitoring the active transport of efflux pumps after their reconstitution into proteoliposomes: caveats and keys.


PMID 22019764

Abstract

There is an acute need for a functional assay allowing the investigation of efflux pumps. A dedicated procedure was previously developed, but although it was unambiguous, it suffered from a lack of reproducibility. We describe an optimization of the procedure that makes the assay much more robust.

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