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Chembiochem : a European journal of chemical biology

Simultaneous purification and site-specific modification of pyrroline-carboxy-lysine proteins.


PMID 22223621

Abstract

Sticky residue: Pyrroline-carboxy-lysine (Pcl) can be readily incorporated into proteins expressed in E. coli and mammalian cells by using the pyrrolysyl tRNA/tRNA synthetase pair. Pcl can be used as a single amino acid purification tag and can be site-specifically modified with functional probes during the elution process.

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