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Bioconjugate chemistry

Novel cyanine dyes with vinylsulfone group for labeling biomolecules.


PMID 22243689

Abstract

Novel vinylsulfone cyanine dyes (em. 550-850 nm) were designed and synthesized for fluorescent labeling of biomolecules via 1,2-addition reaction in aqueous conditions. Due to the virtue of chemical structures of both fluorophore and reactive group, these dyes could be significantly stable and reactive in various aqueous/organic conditions. A wide variety of pH, temperature, buffer concentration, and protein were tested for the optimal labeling condition.

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V3700
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C4H6O2S