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Bulletin of experimental biology and medicine

Optimization of a method for preparation and repopulation of the tracheal matrix for allogenic transplantation.


PMID 22442814

Abstract

A method for decellularization of the trachea for subsequent repopulation with allogenic MSC was optimized. Tracheas from C57BL/6 mice were devitalized and repopulated with MSC from BALB/c mice. The tracheal matrix with devitalized mucosa and intact cartilaginous structure, fit for repopulation with allogenic MSC, was obtained by chemical treatment with NaClO4. This approach seemed to be promising for transplantation of allogenic trachea.

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410241
Sodium perchlorate, ACS reagent, ≥98.0%
ClNaO4