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Applied and environmental microbiology

Expression of bacterial GshF in Pichia pastoris for glutathione production.


PMID 22610434

Abstract

Conventionally, two consecutive enzymatic reactions catalyzed by γ-glutamylcysteine synthetase and glutathione synthetase are most commonly used for glutathione production. Here we demonstrate that bacterial bifunctional GshF can be used for glutathione production in a eukaryotic system without accumulation of the intermediate γ-glutamylcysteine.