The Journal of antibiotics

Microbispora thailandensis sp. nov., an actinomycete isolated from cave soil.

PMID 22781279


The taxonomic position of actinomycete strain NN276(T), isolated from cave soil, was studied using the polyphasic taxonomic approach. A phylogenetic tree based on 16S ribosomal RNA (rRNA) gene sequences showed that the isolate formed a distinct evolutionary linage with the genus Microbispora, with M. mesophila JCM 3151(T) as its closest phylogenetic neighbor (97.9% similarity). The organism contained meso-diaminopimelic acid and the N-acetyl type of peptidoglycan. Madurose was detected in the whole-cell hydrolasate. The predominant menaquinones were MK-9(H(4)), MK-9(H(2)) and MK-9. Mycolic acids were not detected. Major phospholipids were diphosphatidylglycerol, hydroxy-phosphatidylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major cellular fatty acid was iso-C(16: 0) and G+C content 70 mol%. DNA-DNA hybridization demonstrated that the isolate was distinct from M. mesophila JCM 3151(T). On the basis of phenotypic and genotypic data, it is proposed that strain NN276(T) represents a novel species of the genus Microbispora, hence the name Microbispora thailandensis sp. nov. The type strain is strain NN276(T) (=BCC 41490(T)=NRRL B-24806(T)=NBRC 107569(T)).